Abstract

The ultrastructure of Botrytis cinerea Pers. ex Pers. sclerotium primordium cells is similar to that of the surrounding mycelium. Sclerotia from 11 day-old cultures possess a three-layered rind of large, pigmented hyphae and a medulla of loosely arranged hyphae, embedded in a matrix of polysaccharide. The surrounding mycelium starts to degenerate at about 11 days. Rind and medulla cells in 21- and 34-day-old sclerotia contain large vacuoles. The surrounding mycelium is autolysed leaving only the remains of walls and membranes. The inner part of the germinated sclerotium is composed of new germinative mycelium and the remains of medullary hyphae and polysaccharide. The rind hyphae either contain large vacuoles or appear empty and the walls remain well-defined.

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