Abstract
Midterm human fetal sympathetic ganglia were studied using the formaldehyde-induced fluorescence technique for demonstrating catecholamines, and after glutaraldehyde-OsO 4 or permanganate fixation for electron microscopy. The fluorescence technique indicated the presence of catecholamines in most of the developing neurons. After glutaraldehyde-OsO 4 fixation large granular vesicles were found in the perikarya, but a more specific technique for catecholamines, permanganate fixation, revealed no granular vesicles at this stage of development. This indicates that most of the catecholamines are loosely bound in a non-granular store during development.
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