Ultrastructure of extracorporeal secretions of four sessile species of Rotifera (Gnesiotrocha), with observations on the chemistry of the gelatinous tube

Abstract

AbstractSecretion of an extracorporeal hydrogel is a common defensive strategy employed by many aquatic invertebrates including several species of sessile rotifers. Here, we provide a comparative study of the ultrastructure of the gelatinous secretions of four species of Superorder Gnesiotrocha (Monogononta): Conochilus unicornis, Collotheca ferox, Stephanoceros fimbriatus, and Stephanoceros millsii. Additionally, we use differential staining and enzyme degradation assays to explore the chemistry of the gelatinous tube of S. fimbriatus. At least three types of secretions are produced by these four species: an external gelatinous tube, a thick glycocalyx, and an adhesive peduncle. These three different secretions all have a fibrous appearance, but each has different electron densities, fiber thicknesses, and glandular origins. The gel tube, which is likely secreted by pedal glands, has a highly hydrated framework of mesh‐like fibers with thick internal and external laminae that differ slightly among species. Chemical staining suggests that the gel tube is composed of glycoproteins with a high content of sulfonic groups and limited or no obvious lipids or cationic proteins. This tube is resistant to chemical and enzymatic digestion. The proteinaceous glycocalyx is secreted by the syncytial integument and has various densities and thicknesses among species. The peduncle is an adhesive attachment that connects a rotifer's foot to a substratum, or, in some species, to conspecifics as part of a colony. The secretion is highly electron dense and contains membrane‐bound vesicles; it appears to be derived from a different set of pedal glands than the hydrogel tube. The different chemistries and anatomical origins of the three gelatinous secretions suggest an independent evolutionary history for each.