Ultrastructure of dermal fibroblasts during development and aging: Relationship to in vitro senescence of dermal fibroblasts

Abstract

One approach to understanding the relationship between in vitro cellular senescence and in vivo aging is to define the development and aging of cells in vivo and then to compare these in vivo properties with the in vitro behavior of the same cells. The Syrian hamster is being used as an experimental aging model to investigate the intrinsic developmental program of dermal fibroblasts in vivo (proliferation, extracellular matrix (ECM) production, quiescence, and reactivation during wound repair) in order to determine whether the in vivo differentiation program and mature function of these cells is related to their in vitro proliferation and senescence pattern. The ultrastructure of dermal fibroblasts from midfetal development through old age is described, and a working hypothesis of the development and aging of dermal fibroblasts is proposed as a framework for further evaluation of the relationship between in vitro proliferative capacity of dermal fibroblasts and in vivo developmental and age-related changes in the dermis.