Ultrastructure of capillaries in the red body (rete mirabile) of the eel swim bladder

Abstract

The retea mirabilia are paired capillary organs located on the dorsal surface of the swimbladder of the common eel. They consist of bundles of closely apposed capillary segments which function in countercurrent exchange of gases and other solutes and concentrate oxygen in the swim bladder. Ultrastructural features of the afferent arterial capillaries and efferent venous capillaries were studied by scanning EM of corrosion casts and critical-point dried retes and transmission EM of thin sections and freeze-fractured retes. A loose association between endothelial cells in the venous capillaries is indicated by penetration of casting material into interendothelial clefts and the appearance of clefts bounded by cytoplasmic flaps in exposed critical-point dried specimens. In this sections, open gaps between venous endothelial cells are bounded by cytoplasmic processes. Sections through arterial capillaries exhibit tight occluding junctions joining endothelial cells together and these can be seen in freeze-fracture replicas to extend without interruption along the length of the arterial capillaries. These studies indicate the absence of open or hydraulically conductive pathways across the arterial capillary walls and that they probably constitute a rate-limiting barrier in countercurrent exchange of solutes.