Abstract
A new bacteriophage has been isolated and its form has been described. As far as we know, it is active on Bacillus licheniformis only and is called BLE. The phage particles were negatively stained with phosphotungstic acid. The head of phage BLE is bacilliform and is about 50 nm wide and 130 nm in length. The noncontractile tail is 240 nm in length and 6.3 nm thick and has a cross-striated sheath. The base plate of the tail exhibits the shape of a six-pointed star. From the phage BLE, phenol-extracted DNA molecules were prepared for electron microscopy by the cytochrome c film technique of A. Kleinschmidt and R. K. Zahn ( Z. Naturforsch. B, 14, 770–779). Some DNA molecules probably of circular form were observed. Measurement of contour lengths of DNA molecules gave a value 21 ± 0.8 gm, which corresponds to a molecular weight of (41.1 ± 1.5) × 103.
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