Abstract
Five baboons were treated during seven menstrual cycles with 5.0 mg of FSH-P for five days, starting on either day 3 or day 5 of the cycle. On day 5 of the treatment, the ovaries were examined by laparoscopy to evaluate follicular development. All animals exhibited multiple preovulatory follicles and at that time 100 mg GnRH was administered intramuscularly to induce LH release. Between 24 and 30 hours after injection of GnRH, laparoscopic follicular aspiration was used to collect oocytes. These were matured in vitro (determined by extrusion of the first polar body) and fertilized by microinjection with frozen-thawed baboon spermatozoa. Male and female pronuclei were observed in 32% of the resulting zygotes within 24 hours. These zygotes were compared to a zygote in the same stage that had been fertilized in vivo and obtained by laparoscopy and flushing of the oviduct.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
