Ultrastructure of Antennal Sensilla in Adults of Dioryctria rubella Hampson (Lepidoptera: Pyralidae).

Abstract

Simple SummaryThe pine shoot moth (Dioryctria rubella) is a major pest on pine trees in China. It damages the branches and cones of the trees. However, little is known about this pine pest. Identifying the olfactory receptors on its antennae is critical for controlling the moth. Therefore, we studied the different types of sensilla present on the antennae of the pine shoot moth and their morphology using scanning electron microscopy. Results showed that the antennae of the moth were filiform. This form of antennae is common and can be divided into three parts: a basal scape, a pedicel, and a flagellum consisting of flagellomeres. As the sensilla play a vital role in the control of this pest, the research presented is a thorough inventory of sensilla on the antennae of the pine shoot moth. This information is important for further functional studies of the antennae of this pine pest.Antennal sensilla play an essential role in insect life because they receive environmental cues. Dioryctria rubella is an important pine pest in China, but information on the morphology and distribution of its sensilla is limited. To elucidate the mechanism of insect-plant chemical communication, we examined the insect antennae and sensilla by scanning electron microscopy. The results showed that the antennae of D. rubella were filiform and consisted of a basal scape, a pedicel, and a flagellum with tapered flagellomeres. We identified seven types of sensilla, including trichodea, coeloconica, auricillica, basiconica, styloconica (two subtypes), Böhm’s bristles, and squamiformia, all of which were distributed on the antennae of both sexes. Nevertheless, some sensilla exhibited various degrees of sexual dimorphism; for instance, sensilla trichodea, squamiformia, and basiconica were more abundant in males than in females. Many pores were observed on the surface of the cuticular wall in sensilla trichodea and auricillica, and their biological function may be related to olfaction. This study presented a thorough inventory of sensilla on the antennae of D. rubella and laid a solid foundation for future functional studies.