Abstract
In a previous study of amelogenesis in Carcharhinus menisorrah it was demonstrated that enamel crystallites develop within hollow enamel fibrils (tubules), as in mammalian teeth. The long controversy over the origin of the organic matrix of shark tooth enamel has stimulated this investigation of the fine structure of ameloblasts in C. menisorrah and in two other shark species, Triaenodon obesus and Negapri on brevirostris. Tooth tissue of the first two species was collected at Eniwetok Marine Biological Laboratory, Eniwetok Atoll, Marshall Islands; that of Negaprion was donated by Dr. I. Kaufman Arenberg, Washington University, St. Louis, Missouri, who obtained it at the Lerner Marine Laboratory, Bimini, Bahamas. Jaw fragments containing single files of teeth were fixed in phosphate- or cacodylatebuffered glutaraldehyde, postfixed in buffered 1% OSO4 and processed for embedding in epon. Sections were cut with a diamond knife on an LKB Ultratome and stained with uranyl acetate and lead citrate. They were examined with an RCA EMU-3E electron microscope.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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