Abstract
A method is presented for the infiltration and embedding of frozen micro‐organisms at a temperature of 190 K by Nanoplast MUV 116, a new UV‐polymerizable embedding medium for electron microscopy. The hardness achieved by UV irradiation in the cold is about 80% of the hardness needed for ultramicrotomy. Final hardening requires a post‐curing step at 333 K for 24 h.Applying this procedure to cyanobacteria, an improved ultrastructural preservation is demonstrated as compared to samples that were embedded in the water‐soluble medium Nanoplast FB 101 at ambient temperatures. Sections of materials treated in this way are suitable for histochemical techniques, such as Thiéry's silver staining method for glycogen.
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