Ultrastructure and hemodynamics of microaneurysms in retinal vein occlusion examined by an offset pinhole adaptive optics scanning light ophthalmoscope.

Abstract

Retinal microaneurysms (MAs) associated with retinal vein occlusions often cause macular edema due to vascular leakage from the MAs, which can lead to severe vision loss. However, studies using conventional imaging modalities have not shown a significant association between MAs and retinal functional changes. The recent technological advancements to the adaptive optics scanning light ophthalmoscope (AOSLO) have enabled real-time observation of the human retinal microvasculature. Additionally, offsetting the confocal aperture in the AOSLO enables the blocking of specular reflection from the inner retina and the enhancement of the image contrast of the retinal capillaries. This study investigated the ultrastructure and hemodynamics of MAs examined by structural images and perfusion maps of the offset pinhole AOSLO and evaluated their associations with vascular leakage on fluorescein angiography. Our results show the diverse configurations of the MAs, some of which are occasionally accompanied by a cap structure on the aneurysmal surface. Moreover, the morphological and hemodynamic changes were significantly associated with vascular leakage.