Ultrastructural studies of late-stage spermatids and mature spermatozoa of the puffer fish, Takifugu niphobles (Tetraodontiformes) and the effects of osmolality on spermatozoan structure

Abstract

Ultrastructures of late-stage spermatids and spermatozoa, and of spermatozoa after exposure to various osmolalities, were studied in the puffer, Takifugu niphobles. The mature spermatozoa consisted of a head, a midpiece of many mitochondria and a flagellum with sharp sidefins, had many ring-structures just inside of the plasma membrane of cytoplasmic sleeve and triangular-structures projecting into cytoplasmic canal at the base of flagellum. In late spermatids, the rings and projections were present, but the side-fins had round ends and the cytoplasm of flagellum was amorphous. When spermatozoa were exposed to seawater, the plasma membrane became swollen in the head-midpiece region but shrank in the tail region. In 1/2 seawater, swelling in the tail occurred in some spermatozoa. In 1/3 seawater approximately isotonic to the seminal plasma, there was little change. In 1/10 seawater, the plasma membrane swelled slightly in the head region, but swelled much more in the tail region. In buffer solution, the membrane swelled in all regions, surrounding the nucleus and many sections of axoneme. Thus, function of the plasma membrane in the head-region may be different from that in the tail-region. Spermatozoa of marine fish may fertilize the eggs when the osmolality surrounding the sperm, which changes due to the mixing of seminal plasma and seawater, reaches the correct level for the spermatozoa to obtain correct structure.