Abstract

Allogromia sp. (strain N.F. Lee) provides a convenient model system for studying intracellular transport. We have been examining ultrastructural changes which occur in the reticulopodial network (RN) and cell body (CB) of Allogromia at various stages in the retraction response stimulated by 0.4M MgCl2 solution. As retraction proceeds, microtubules (MT) disassemble and form areas of regularly packed cytoplasm (TP-tigroid patterning) resembling vinca alkaloid induced paracrystals of tubulin. TP is first seen in the RN, and later in the CB. TP consists of dark filamentous material 12.5 nm in width separated by a light spacing of 8 nm when plane of section gives a perfect repeat pattern (arrow-fig. 6). TP may fill the entire profile of a reticulopod and in early stages of retraction is seen in close juxtaposition to MT. In the CB, TP occurs in patches 0.3-2.0 μm in diameter. TP is seen with great frequency in retracting Allogromia, but also, it can be seen occasionally in animals with fully extended RN.

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