Abstract
γ-Tubulin is an essential component of the microtubule nucleation-organizing center (MTOC) and plays a crucial role during mitosis and differentiation. Because the γ-tubulin content of individual cells is very low, we relied principally on the high resolution and sensitivity afforded by immunoelectron microscopy and used polyclonal antibody against γ-tubulin in undifferentiated and induced differentiated human promyelocytic leukemia cells (HL-60) by 1 μM all-trans retinoic acid. The effectiveness of this approach was demonstrated by the distribution of γ-tubulin from differentiated HL-60 cells relative to undifferentiated cells. Our studies revealed that, like the situation in undifferentiated cells, γ-tubulin labeling is mostly found in the pericentriolar region, with a few instances in the cytoplasm. However, in the differentiated cells, serial immuno-gold analysis indicated that the amount of γ-tubulin in noncentrosomal region of cytoplasm was almost greater than three times the amount of γ-tubulin in undifferentiated cells (The J Histotechnol 30:35, 2007).Submitted November 21, 2006; accepted with revisions January 17, 2007.
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