Abstract
Intimal injury is considered as one of the initiation factors of atherosclerosis and intimal thickening. However the morphological elucidation after intimal injury is not so clear. On the other hand, fibronectin is a glycoprotein that play an important role in cell adhesion, migration, spreading, and differentiation. This study was designed to examine the mechanism of serial intimal regeneration after intimal injury and the localization of fibronectin using scanning and transmission electron microscope.Male Wistar rats, weighing 400g were used and endothelial denudation was produced by the modified methods of Baumgartner. A deflated vascular balloon catheter( 1.5F, DOW CORNING , Corp.) was introduced into the thoracic aorta, then inflated up to 2.5mm in diameter, and passed through the aorta three times to a length of 2.5cm. The animals were sacrificed at 15min, 3,7,14 and 28 days after denudation. For electron microscopic examination, the tissues were fixed in 2.5% glutaraldehyde in 0.1M phosphate buffer, treated with 1% osmium tetroxide in phosphate buffer, and dehydrated in a graded ethanol series.
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