Abstract
Photooxidation of diaminobenzidine (DAB) has been used to detect ultrastructurally the photosynthetic activity (photosystem I) in the thylakoids of etiochloroplasts in greening bean leaves. The result of this photooxidation is a dark (osmiophilic) deposit which accumulates at first in certain portions of the primary thylakoids. In the course of further greening this area enlarges more and more and at last all the thylakoids become uniformly dark. It has been shown that the beginning of the appearance of the DAB deposits and the speed of their accumulation in the thylakoids largely depends on the experimental conditions of the plants: in leaves maintained in damp atmosphere the first DAB deposits appear between the first and the second hour of greening, while in those kept in dry air this does not happen until three or more hours in light.
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