Ultrastructural localization of 2,4-dinitrophenyl groups on draining lymph node cells of guinea pigs following skin painting with 2,4-dinitrochlorobenzene: I. Scanning immunoelectron microscopic studies.

Abstract

ABSTRACTThe distribution of DNP groups on cells taken from draining lymph nodes of guinea pigs 12 hours after painting the skin with DNCB was examined by scanning immunoelectron microscopy using antibody directed against DNP groups with bacteriophage T4 as a visual marker. The studies showed that DNP groups were distributed on the surface of the cells, demonstrating some differences in distribution pattern among the cells. The incidence of cells on which DNP groups were detected was approximately 60% in the normal animals, higher than that detected by a previous immunofluorescent study. The incidence was markedly reduced to approximately 3% by pretreatment with tolerogen, DNBSO3Na. DNP groups were detected on the surface of the cells forming both rabbit red cell rosettes and complement coated red cell rosettes. It is suggested that DNCB penetrates through the skin into the lymphatics carrying lymph from the painted site and combines with cell membrane components in the peripheral lymphoid system.