Ultrastructural changes in glial cells during regeneration of cockroach peripheral nerve

Abstract

After peripheral nerve 5 in the cockroach Periplaneta americana was cut, changes occurring in the glial cells in the proximal stump were studied immediately after damage and during the process of nerve regeneration. During the first week haemocytes accumulated outside the nerve and morphologically similar granule-containing cells appeared inside the nerve. These cells were involved in phagocytic activity. Between the second and the fourth week, signs of regeneration were distinguishable; many small axonal sprouts were formed which were surrounded by glial processes, and the nerve stump increased in length. During this period the glial cells produced large amounts of extracellular material in which the bundles of axons and glia were embedded. The structural differences between glial and perineurial cells were lost during these stages of regeneration and there was no restriction to the penetration of the extracellular tracer lanthanum. After 8 weeks, reinnervation of the muscles had taken place, perineurial and glial cells were again distinguishable, and the perineurial cells were able to exclude lanthanum.