Abstract
The cytological changes that occur during the process of T-cell-mediated cytolysis (CMC) were studied in correlation with the 51Cr release assay. Our data show that both target P-815 and the effector cytolytic T-lymphocytes (CTL), when adequately purified, have a well-defined and distinguishable surface morphology which remained unaltered after lymphocyte-target cell binding. At subsequent stages of the CMC process when specific 51Cr release became significant, some target cells lost their surface microvilli and developed large surface protrusions or blebs. CTL remained bound to target cells undergoing the blebbing process with only minor changes in their surface morphology. Quantitation of the cellular interactions revealed that the frequency of target cells blebbing and binding of specific CTL rose concomitantly with the specific 51Cr released. Transmission electron microscopy indicated that the earliest cytological damage occurred apparently in the mitochondria of the target cells at a stage of the CMC process preceding significant 51Cr release.
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