Ultrastructural and Histochemical Studies of the Cells Producing the Gelatinous Matrix in Meloidogyne

Abstract

The fine structure of the cells producing the gelatinous matrix which envelops the eggs of Meloidogyne javanica, has been studied at various stages of development, together with the secreted matrix itself. At the peak of matrix production these cells have a dense cytoplasm containing numerous mitochondria, Golgi bodies and multivesicular lamellar bodies, together with sinus canals which lead to the exterior. The nucleus is large, with a nuclear envelope which is difficult to define when stained with osmium and permanganate, but which can be seen more clearly after lead staining. Histochemical tests on these cytoplasmic structures show that they contain protein, acid-mucopolysaccharide and traces of alkaline phosphatase, acid phosphatase and lipid. Observations on the morphology of these structures suggest a relationship between Golgi bodies, multivesicular lamellar bodies and the secretion of the gelatinous matrix. A study of sections of the gelatinous matrix under the electron microscope reveals that it has a mesh-like appearance.