Abstract
The chemical and ultrastructural architecture of the cell wall of Epidermophyton floccosum was investigated by extraction of the purified cell walls with alkali and/or acid, then by analysing the residues and extracts by gas liquid chromatography, X-ray diffraction and electron microscopy. The cell wall of E. floccosum appeared to be two layers: microfibrils and an amorphous matrix. The microfibrils as observed here are composed of chitin and a glucan in the core, and an alkali-insoluble, acidsoluble polysaccharide enveloping the chitin core. The amorphous matrix was also observed as a fine granular material, which is an alkali-soluble, 4 N-acetic acid-insoluble polysaccharide containing glucose, mannose and galactose. A schematic model of the cell wall is proposed and discussed.
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