Abstract
Background: Ultrasonic extraction is one method for optimization of effective compounds from plants. Objectives: The aim of this study was optimization of the extraction conditions of Myristica fragrans seeds using ethanol as a food grade solvent and by ultrasonic-assisted extraction using response surface method (RSM). Methods: Ultrasonic-assisted extraction was used for extraction of M. fragrans seeds. Response surface methodology (RSM) was used to optimize three parameters: time and of temperature extraction (min, °C), and solvent to material ratio. Antioxidant activity and phenolic contents were determined. Results: The results showed that optimal ultrasonic-assisted extraction (UAE) conditions were 29.57 minutes extraction time, 374.61 mL/g ratio of solvent to material and 41.89oC extraction temperatures. The yield of extraction, amount of total phenolic content, IC50 (in DPPH method) and FRAP in M. fragrans seeds in optimum conditions were 18.14%, 63.41 mg gallic acid/g of extract, 43.66 mg/mL and 99.38 mmol/g of extract, respectively. Conclusions: The results indicated of the ultrasonic-assisted extraction having power for optimization of the extraction condition and it is appropriate for extraction of antioxidant and phenolic compounds.
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