Abstract
The conventional polymers (polyethylene, polypropylene, polystyrene, etc) are non-degradable and exhibit many hazardous effects on the environment. Due to their bio incompatibility and accumulation in nature, there is a need to produce environment-friendly biodegradable polymers. Polyhydroxybutyrate is one of the biodegradable biopolymers produced by a microorganism Cupriavidus necator in order to survive the stress condition. In this study, the effect of ultrasound to intensify the productivity of biopolymer was studied. Different ultrasound parameters, mainly the effect of ultrasound power, duty cycle, ultrasound irradiation time on the productivity of PHB were studied using the one-factor-at-a-time method. Intracellular PHB was extracted using chloroform as a solvent, and analysis was carried out using GC–MS, FT-IR analytical techniques for the detection of PHB. The highest productivity of 0.2155 ± 0.0049 g/100 mL (1.7 g PHB g−1 dry cell wt−1) is reported when a substrate was irradiated with ultrasound at 22 kHz frequency, 50 W power, 10% duty cycle and 10 min of irradiation time with a constant cycle time of 1 min.
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