Ultrasound-assisted extraction of sucrose, glucose, and fructose from roselle seeds

Abstract

Roselle seeds were utilized as a resource to extract saccharides. The components of the seeds extracted with water were characterized by gas chromatography–mass spectrometry (GC–MS). The result showed that the monosaccharides fructose, glucose, and galactose were extracted. The ultrasound-assisted extraction of saccharides was performed at different frequencies of 26, 78, and 130 kHz. For comparison, the conventional mixing method was used for the extraction. The effect of frequency on the total amount of extracted saccharides was unclear. However, the obtained results showed the existence of an enzymatic system that converts sucrose to fructose and glucose. The positive effect of ultrasound on sucrose hydrolysis was observed at 26 kHz. The components extracted with methanol were also analyzed by GC–MS to determine the existence of this enzymatic system in water. By comparing the components extracted by water with those extracted by methanol, the progress of the hydrolysis of the disaccharide to the monosaccharides in water was confirmed.