Ultrasound assisted extraction of polysaccharides from mushroom by-products

Abstract

The effect of ultrasound technology to extract the water soluble polysaccharides from dried and milled by-products generated from Agaricus Bisporus production was studied. Amounts of β-glucan 1.01 and 0.98 g/100 g dry mass were obtained in particle sizes of 355–250 μm and 150-125 μm from the mushroom by-products. Three parameters of extraction were studied; extraction time (0–15 min), ultrasonic amplitude (20–100 μm) and precipitation time (1 or 18 h). The application of ultrasounds enhanced the extraction polysaccharide yields compared to the untreated samples. The highest extraction yield of 4.7% was achieved with an extraction time of 15 min, maximum amplitude of 100 μm with 1 h of precipitation in 80% ethanol. The coefficient of determinations for predicted water soluble polysaccharides extraction yields showed good correlation with the experimental data at the 95% confidence level and indicated that the non-exponential Peleg's model could be employed to predict the extraction polysaccharide yields after ultrasound treatment.