Ultrasound-Assisted Extraction of Anthocyanins from Haskap (Lonicera caerulea L.) Berries Using a Deep Eutectic Solvent (DES) DES Extraction of Anthocyanins from Haskap Berries.

Amanda M.G Maclean,Guangling Jiao,Marianne S Brooks,Yasmini P.A Silva

doi:10.17113/ftb.59.01.21.6869

Amanda M.G Maclean, Guangling Jiao + Show 2 more

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https://doi.org/10.17113/ftb.59.01.21.6869

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Abstract

SUMMARYResearch backgroundHaskap berries are one of the richest natural sources of anthocyanins and their extracts can be used for nutraceuticals and functional food ingredients. Deep eutectic solvents (DES) comprising food-grade or generally recognized as safe (GRAS) components show promise as natural solvents, but have not been applied to haskap berries. Thus, the aim of this study is to investigate the extraction of anthocyanins from haskap berries using a DES consisting of citric acid and d-(+)-maltose.Experimental approachThe experimental approach used ultrasound-assisted extraction with a DES consisting of citric acid and d-(+)-maltose as the solvent to achieve a sustainable green extraction process. Response surface methodology (RSM) with a Box-Behnken experimental design was used to study the effect of varying the extraction temperature, time of extraction, V(solvent)/m(sample) ratio (mL/g) and the water volume fraction (%) in the DES on the total anthocyanin content (TAC) in the haskap berry extracts.Results and conclusionsUnder the optimal extraction conditions (75 °C, 10 min, 50.4 mL/g and 90% water) a predicted TAC extraction on dry mass basis yielded 21.2 mg/g, with experimental error of 7.2%. The TAC yield and anthocyanin profiles were similar to those obtained with conventional organic solvents.Novelty and scientific contributionThis is the first study investigating the use of a food-grade DES comprising GRAS components for the extraction of anthocyanins from haskap berries. These results indicate that the studied DES (citric acid and d-(+)-maltose) is a suitable alternative solvent for extracting anthocyanins for food-grade applications.

Highlights

Phone: +1(902)494 6482Haskap (Lonicera caerulea L.) berries, a fruit native to Siberia and northeastern Asia, have recently entered the North American market [1] and are known for their high anthocyanin content [2]
The objective of this study is to investigate the ultrasound-assisted extraction (UAE) of anthocyanins from haskap berries using a Deep eutectic solvents (DES) prepared of citric acid and d -(+)-maltose as an alternative, sustainable, food-grade solvent
Citric acid is an antioxidant and could add to the antioxidant activity of the bioactive compounds in the extract and the final food product. This is the first investigation of anthocyanin extraction from haskap berries using a deep eutectic solvent (DES)

Summary

Introduction

Phone: +1(902)494 6482Haskap (Lonicera caerulea L.) berries, a fruit native to Siberia and northeastern Asia, have recently entered the North American market [1] and are known for their high anthocyanin content [2]. Anthocyanins are flavonoids associated with antioxidative, anti-inflammatory, and anticarcinogenic properties [3], and are highly desirable for their application as dietary supplements and natural colourants [4]. They are extracted from natural sources using traditional solvents, such as methanol and ethanol [5]. The extraction of bioactive compounds from natural sources can be made more environmentally friendly with assisting technologies such as ultrasound to improve extraction efficiency [6] and the development of green solvents. Deep eutectic solvents (DES) can be produced by mixing two or more natural components capable of hydrogen bond interactions [8]. The extraction of anthocyanins from grape skin [12,13], wine lees [14] and Lycium ruthenicum Murr. fruit [15] using various DES has been reported

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