Abstract
Box-Behnken design (BBD), one of the most common response surface methodology (RSM) methods, was used to optimize the experimental conditions for ultrasound-assisted extraction of polysaccharides from Rhynchosia minima root (PRM). The antioxidant abilities and anticancer activity of purified polysaccharide fractions were also measured. The results showed that optimal extraction parameters were as follows: ultrasound exposure time, 21 min; ratio of water to material, 46 mL/g; ultrasound extraction temperature, 63 °C. Under these conditions, the maximum yield of PRM was 16.95% ± 0.07%. Furthermore, the main monosaccharides of purified fractions were Ara and Gal. PRM3 and PRM5 exhibited remarkable DPPH radical scavenging activities and reducing power in vitro. PRM3 showed strong inhibitory activities on the growth of MCF-7 cells in vitro. The above results indicate that polysaccharides from R. minima root have the potential to be developed as natural antioxidants and anticancer ingredients for the food and pharmaceutical industries.
Highlights
Ultrasound-assisted extraction, by means of ultrasonic vibration, can facilitate the release of components from sample matrices [1]
Multiple literatures have reported that ultrasound-assisted extraction with Response surface methodology (RSM) is commonly applied in analyzing bioactive components of plants, such as Chrysanthemum morifolium flower heads [6], Ligusticum rhizomes [7], sweet basil [8], and sugar beet [9]
AtIn8 amdgd/itmionL, tthhee pICer50cevnatlaugeess ooff PscRaMve1n, gPiRnMg 3foranPdRMPR1M, P5RwMe3rean3d43P.2R4,M254.w32eraend6.5153%.9,82m6.5g3/m%La, nd 35r.e1s3p%ec, trievseplye.cTtihvieslyr.esIunltasdidnditiicoant,edthtehIaCt 5P0RvMa5luceosuoldf PsuRpMp1ly, PmRoMre3haynddroPgRenMa5towmesreth3a4n3.P2R4,M214.a3n2dand 13P.9R8Mm3g[1/5m].L, respectively. This results indicated that PRM5 could supply more hydrogen atoms than PRThMe1readnudcPinRgMp3ow[1e5r].assay reveals the electron donating activity and can be used to evaluate the potential antioxidant activity of polysaccharide fractions [16,17]
Summary
Ultrasound-assisted extraction, by means of ultrasonic vibration, can facilitate the release of components from sample matrices [1]. Response surface methodology (RSM), a validated collection of statistical and mathematical techniques, is commonly used to optimize and evaluate complex experimental process factors and their interactions [4]. Multiple literatures have reported that ultrasound-assisted extraction with RSM is commonly applied in analyzing bioactive components of plants, such as Chrysanthemum morifolium flower heads [6], Ligusticum rhizomes [7], sweet basil [8], and sugar beet [9]. IInn oorrddeerr ttoo ffuurrtthheerr ddeevveelloopp aanndd eexxpplloorree tthhiiss vvaalluuaabbllee rreessoouurrccee,, tthheerree iiss aa pprreessssiinngg ddeemmaanndd ttoo oobbttaaiinn eexxtteennssiivvee ccrruuddee ppoollyyssaacccchhaarriiddeess iinn aa sshhoorrtt ssppaann ooff ttiimmee. AAnnttiiooxxiiddaanntt aaccttiivviittyy aanndd aannttiiccaanncceerr aaccttiivviittyy ooff ppuurriififieedd ffrraaccttiioonnsswweerreeaallssooeevvaalluuaatteeddiinnvviittrroo
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