Abstract
Actinidia arguta pollen owns abundant nutrients, such as vitamins, polyphenols, etc., however, little research on its antioxidant ability and biological function was conducted. In this study, we observed A. arguta pollen spore structure by SEM (Scanning electron microscope), analyzed the phenolic composition of A. arguta pollen extract (AAPE) obtained by four extraction methods (A: ultrasound-assistedextraction with water, B: heatrefluxextraction withwater, C:ultrasound-assistedextraction with ethanol, and D: heatrefluxextraction withethanol). Total phenolic content, total flavonoid content, antioxidant activities (ferric reducing/antioxidant power [FRAP], chelating activity, and DPPH⋅ scavenging activity) were also determined. Finally, we investigated its protective effect on DNA and lymphocytes damage response to oxidative stress. The results showed that the morphology of A. arguta pollen was similar to other pollen of the genus A. Lindl., but differs from them slightly in the specific morphology indicators. What is more, AAPE obtained by different extraction methods exhibited a protective effect against DNA oxidative damage, they also possessed a strong cytoprotection effect on mouse lymphocytes, especially the extraction obtained by method C, which had the highest total phenolic content (15.05±0.34mg GAE/g), strong ferrous ion-chelating ability (0.37±0.023mg Na2 EDTA/g), DPPH⋅ scavenging activity (IC50 =0.14±0.04mg/ml), and FRAP (7.13±0.33mg Trolox/g). This paper provided a new edible natural antioxidant for human which will protect us from oxidative stress. PRACTICAL APPLICATIONS: This study was the first to report the morphology of Actinidia arguta pollen by SEM analysis, and studied the effects of different extraction methods on antioxidant activities and cytoprotective effect of A. arguta pollen extract. We believed that our research makes a significant contribution to the literature, because the results of this research provide a reference for the development of a new edible natural antioxidant.
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