Abstract
A flower-like nanobioreactor was prepared for resolution of ibuprofen in organic solvents. Ultrasound irradiation has been used to improve the enzyme performance of APE1547 (a thermophilic esterase from the archaeon Aeropyrum pernix K1) in the enantioselective esterification. Under optimum reaction conditions (ultrasound power, 225 W; temperature, 45 °C; water activity, 0.21), the immobilized APE1547 showed an excellent catalytic performance (enzyme activity, 13.26 μmol/h/mg; E value, 147.1). After ten repeated reaction batches, the nanobioreactor retained almost 100% of its initial enzyme activity and enantioselectivity. These results indicated that the combination of the immobilization method and ultrasound irradiation can enhance the enzyme performance dramatically.
Highlights
Ibuprofen is a nonsteroidal drug and has an asymmetric carbon in its second position
Our unpublished has demonstrated that this kind study, we combined this immobilization method. We combined this immobilization method and ultrasound tohigh improve of immobilized enzyme is very stable under many extreme environments
We prepared a flower-like nanobioreactor for resolution of ibuprofen in organic solvents under ultrasound irradiation
Summary
Ibuprofen is a nonsteroidal drug and has an asymmetric carbon in its second position. In 2014, we prepared a flower-like nanobioreactor which of immobilized enzyme is very under many extreme environments (low or high pH, high enzyme is very stable under manystable extreme environments pH, high temperature, organic was formed via the coordination between Cu2+ and the nitrogen atoms of the amide groups in the temperature, organic reaction media, high-speed agitation or ultrasound, et al.). We combined this immobilization method and ultrasound tohigh improve of immobilized enzyme is very stable under many extreme environments Temperature, organic reaction media, high-speed agitation or ultrasound, et al.) These promising the advantages make this of immobilization method more attractive for further under study.
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