Abstract
Chitosan nanoparticles have been prepared using an ionic gelation method. The effects of freeze-drying, cryoprotectant (d-trehalose) utilisation and ultrasonication time on the mean particle size, size distribution and stability of nanoparticles were investigated. The size distribution results indicated that freeze-drying caused the particles to agglomerate. Addition of the cryoprotectant and ultrasonication led to a decrease in the particle size. However the sizes of the nanoparticles increased with increasing ultrasonication time. Transmission electron microscopy images revealed that spherical chitosan nanoparticles ranging from 3 to 12nm were obtained by means of freeze-drying in the presence of cryoprotectant and ultrasonication. These results suggest that cryoprotectant utilisation and controlled ultrasonic treatment are effective for imparting nanoparticle stability.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
