Abstract
A new method was developed to determine the oil and grease in water by HPLC-Vis fluorescence technique. Ultrasonication liquid-liquid extraction method was applied by using 1 ml of toluene to 100 ml of water. The analysis was carried out on a LC-10AT-VP chromatograph with a fluorescence RF. 10 a syknm detector equipped with a varian CN (250 × 4.6 μm) Column. The separation was preformed isocratically using a mixture of 90% solvent (n-Hexane) with 10% solvent (Toluene). Asphaltenic compounds were established to be the main fluorescence chromophores (excitation wave length at 420 nm and emission wave length at 500 nm). The correlation coefficient was (0.992), recovery ranged from 92% to 108% and the limit of detection was found to be 0.06 μg.L-1. The method can be utilized successfully for determination of oil and grease in water samples.
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