Abstract
The ultrasonic parameters were carried out at a frequency of 2 MHz using pulse echo overlap (PEO) technique on Escherichia coli grown in nutrient broth at a constant temperature of 37°C. The values of acoustic impedance ( Z), isotropic compressibility ( K s) and attenuation coefficient ( α) were calculated from measured values of ultrasonic velocity ( v), echo-amplitudes ( a n ) and density ( ρ). The ultrasonic velocity values were correlated with Jacobson's [Acta Chemica Scandi. 5 (1951) 1214 and 6 (1952) 1927] free length theory (FLT). The variation of ultrasonic parameters with the time of growth of bacteria has been interpreted on the basis of motility, shape and size of the bacteria in this publication.
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