Ultrasonic examination of differences in bone density of chronic obstructive pulmonary disease (COPD) rats constructed by different methods.

Abstract

Osteoporosis is one of the main complications of chronic obstructive pulmonary disease (COPD) patients. We investigated the bone density in rat model of COPD induced by different method. We developed seven distinct groups to evaluate the differences in bone density. Based on these seven different ways, rats were categorized into distinct groups in which simple cigarette inhalation, sulfur dioxide inhalation, lipopolysaccharide intubation, protease induction, bacterial nasal injection, smoking combined with endotoxin, smoking combined bacterial infection, and smoking combined with protease were tested. The results from the male Sprague-Dawley (SD) rats were used to construct the COPD model, and then the bone density of the rats' right femurs was examined by the ultrasonic bone density testing. The compound factor-induced COPD rat model provides a better simulation of the clinical characteristics and pathophysiological processes of COPD. Smoking combined with bacterial infection method has the most apparent effect on reducing bone density, with statistical differences (P<0.05). The COPD rat model constructed by the combination of smoking and bacterial infection method has a much lower bone density.