Ultrasonic communication in rats: Effects of morphine and naloxone on vocal and behavioral responses to playback of 50-kHz vocalizations

Abstract

Rats emit ultrasonic vocalizations and it was hypothesized that these vocalizations have an important role in intra-specific communication. Recently, we demonstrated that playback of 50-kHz ultrasonic vocalizations can induce social approach, indicating that 50-kHz calls can serve to (re)establish or to maintain social contact. It is known that endogenous opioids are implicated in the regulation of social behavior, particularly in rough and tumble play. Here, we tested whether administration of opioid ligands can affect social approach in response to playback of 50-kHz calls in juvenile and adult rats. Rats were either treated with 1 mg/kg naloxone, 1 mg/kg morphine, or with saline vehicle. Administration of opioid ligands affected social approach at both ages. Specifically, in juvenile and adult rats, social approach displayed in response to playback of 50-kHz calls was reduced in case of naloxone treatment, but enhanced with morphine. Furthermore, juvenile rats treated with saline or morphine emitted a substantial amount of ultrasonic vocalizations in response to the playback of 50-kHz calls. Such ultrasonic calling was not seen in naloxone treated rats. Importantly, these drug-dependent differences were stimulus-specific, i.e. seen only in response to playback of 50-kHz calls and not in response to playback of background noise. The present finding that opioid ligands can affect social approach and ultrasonic vocalizations induced by playback of 50-kHz calls, indicates that an important feature of social interaction in rats, namely ultrasonic communication, is at least partially regulated by endogenous opioids.