Abstract
Based on the necessity and urgency of detecting infectious disease marker procalcitonin (PCT), a novel unlabeled photoelectrochemical (PEC) immunosensor was prepared for the rapid and sensitive detection of PCT. Firstly, SnO2 porous nanoflowers with good photocatalytic performance were prepared by combining hydrothermal synthesis and calcining. BiOI nanoflowers were synthesized by facile ultrasonic mixed reaction. Ag2S quantum dots were deposited on SnO2/BiOI composites by in situ growth method. The SnO2/BiOI/Ag2S composites with excellent photoelectric properties were employed as substrate material, which could provide significantly enhanced and stable signal because of the energy level matching of SnO2, BiOI and Ag2S and the good light absorption performance. Accordingly, a PEC immunosensor based on SnO2/BiOI/Ag2S was constructed by using the layered modification method to achieve high sensitivity analysis of PCT. The linear dynamic range of the detection method was 0.50 pg·mL−1~100 ng·mL−1, and the detection limit was 0.14 pg·mL−1. In addition, the designed PEC immunosensor exhibited satisfactory sensitivity, selectivity, stability and repeatability, which opened up a new avenue for the analyzation of PCT and further provided guidance for antibiotic therapy.
Highlights
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Indium tin oxide (ITO) glasses were obtained from Zhuhai Kaivo Electronic Components
ITO glasses were successively washed in acetone, ultrapure water, ethanol and ultrapure water under ultrasound before being used as working electrode
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Due to its high accuracy for the diagnosis of bacterial infections, procalcitonin (PCT). Is an FDA-approved blood infection marker for guiding antibiotic therapy [1,2]. The level of PCT is less than 0.1 ng·mL−1 [3]. When the human body suffers from bacteria, fungi, or parasites and more severe sepsis or multiple organ failure, the level of PCT increases sharply. When PCT concentration in serum exceeds 0.5 ng·mL−1 , the antibiotic therapy will be strongly recommended [4].
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