Abstract
We propose an ultrasensitive leaky surface acoustic wave (LSAW) immunosensor based on molybdenum disulfide @ cuprous oxide—gold (MoS2@Cu2O-Au) nanoparticles and subsequent gold staining for the detection of alpha-fetoprotein (AFP). MoS2@Cu2O-Au nanoparticles, with their large specific surface area and good biocompatibility, not only capture the secondary antibodies (Ab2) but also amplify the mass loading effect of the acoustic wave sensor in the detection of AFP. The immunosensor signals are further amplified upon injection of gold staining solution. The developed immunosensor achieved a low detection limit of 5.5 and 25.0 pg/mL with and without gold staining, respectively. The immunosensor demonstrated its efficiency for the quantitative detection of AFP in complex biological fluids, including human serum and saliva samples, with excellent selectivity and long-term stability, showing great potential for the quantification of AFP in clinical diagnosis.
Highlights
The scanning electron microscopy (SEM) and TEM images of as-synthesized MoS2 exhibited a flower-like morphology with a large surface area (Figure 3B,E)
Upon the addition of Cu2O nanocrystals to the solution during MoS2 nanoflower synthesis, Cu2O nanocrystals were dispersed with MoS2 nanoflowers, which results in the formation of coral-like MoS2@Cu2O nanohybrids
Sandwich-type leaky surface acoustic wave (LSAW)
Summary
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cancer poses a serious threat to human health worldwide, and its early diagnosis has the potential to reduce mortality rates. The detection of tumor markers is an effective approach for the early diagnosis of cancer [1]. Alpha-fetoprotein (AFP), an embryo-specific glycoprotein, is a widely accepted biomarker for fetal defects and tumor progression
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
