Abstract
A novel ultrasensitive, highly selective immunoassay method that uses gold ultrafine particles as the labeling material and laser-induced photothermal beam deflection quantitation is proposed. Ultrasensitive laser spectrometric analysis of actual biological samples is always obstructed by the background and its fluctuation. However, we could adequately suppress this obstruction by substituting polystyrene microspheres in the antigen-antibody solid-phase reaction instead of the conventional nitrocellulose membrane because the nonspecific adsorption velocity of the admixture proteins was highly restrained and the area of the antigen-antibody reaction was increased
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