Year-end Sale % OFF 
Abstract
A novel highly sensitive and selective electrochemical sensing system based on DNAzyme and rolling circle amplification (RCA) for the determination of Pb2+ was developed in the present study. Firstly, the DNAzyme catalytic strands were immobilized onto magnetic beads surface and then hybridized with substrate strands. In the presence of Pb2+, the DNAzyme could be activated to cleave the substrate strand into two DNA fragments. After RCA reaction, a long ssDNA product with repeating sequence was obtained. Subsequently, CdS QDs modified ssDNA (CdS QD-ssDNA) were used as signaling probes to hybridize with the long ssDNA product. Due to the dramatic signal amplification by the numerous QDs and the low background signal by magnetic separation, ultra-low level (7.8pM) of Pb2+ could be detected. Furthermore, with the application of Pb2+ dependent DNAzyme, the proposed sensing system exhibited high selectivity. The proposed sensing system showed a promising potential for on-site testing and would gain wide applications in real sample analysis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
