Abstract
Our concerns have been raised about whether prolonged exposure to ammunition-related chemicals could correlate with genomic instability predisposing to lung carcinogenesis. The group of professional soldiers engaged in routine ammunition analysis and its explosive properties testing. To assess the presence of an innate genetic profile, DNA isolated from swabs was analyzed with LungCarta and HS Lung Panels and MassARRAY Analyzer 4 mass spectrometry. The presence of new somatic single nucleotide polymorphisms (SNPs) was detected with liquid biopsy technique and circulating cell-free DNA (ccfDNA) isolation, which was further analyzed with UltraSeek Lung Panel. Both genetic profiles obtained for each individual were compared. Genetic analysis revealed that in professional soldiers with long-term exposure to ammunition-related toxic chemicals, new SNPs in genes related to lung carcinogenesis are present. The preliminary data indicate that using modern molecular techniques could be a valuable tool for monitoring the genome instability in context of occupational risk of harmful volatile toxic compounds and improving personnel safety. The analyzed group will be further enlarged, and follow-up studies will be continued.
Highlights
One of the critical research tasks to assess the adverse health effects of exposure of professional soldiers to chemical, physical, and biological agents in the place of service is implementing modern molecular diagnostic methods and identifying threats that could predispose to their occurrence.Communicated by Michal WittMolecular diagnostics of DNA integrity and stability, enriched with the assessment of proteome homeostasis and inflammatory markers, allows for early detection of adverse changes at the cell level
The presence of new somatic single nucleotide polymorphisms (SNPs) was detected with liquid biopsy technique and circulating cell-free DNA isolation, which was further analyzed with UltraSeek Lung Panel
Genetic analysis revealed that in professional soldiers with long-term exposure to ammunitionrelated toxic chemicals, new SNPs in genes related to lung carcinogenesis are present
Summary
One of the critical research tasks to assess the adverse health effects of exposure of professional soldiers to chemical, physical, and biological agents in the place of service is implementing modern molecular diagnostic methods and identifying threats that could predispose to their occurrence. Molecular diagnostics of DNA integrity and stability, enriched with the assessment of proteome homeostasis and inflammatory markers, allows for early detection of adverse changes at the cell level. In the presented research, using the conducted at the Kaczkowski Military Institute of Hygiene and Epidemiology catalog of harmful and carcinogenic substances present in the workplaces of soldiers, engaged in the experimental characterization of explosives (under National Health Program 2016–2020), we identified and assessed the effect of selected chemical factors on genomic stability, examined by the presence of point mutations in a selected set of genes. Genetic analysis was performed using the highly advanced MALDI TOF MassARRAY Analyzer 4 (Agena Bioscience, USA) spectrometer with dedicated genes panels, which identifies the presence of point mutations with high sensitivity and accuracy. In our opinion, promising perspective of routine diagnostic of elite professional groups using advanced molecular methods
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