Ultramicroscopy – a novel light sheet based imaging technique created by various research disciplines

Abstract

Ultramicroscopy (UM) is an interdisciplinary imaging technique allowing three dimensional reconstruction of up to cm-sized organs of animal models with µm resolution. Here, we present a review of technical and theoretical aspects of standard laser light sheet fluorescence microscopy. Fine morphological details in structures of animal models such as Drosophila melanogaster (fruit fly) and GFP-expressing mice, produced by UM are presented.