Abstract
The ultramicrodetermination of cyanocobalamin (9 ng/g) in elemental diet containing 46 kinds of compounds, with concentrations at least 50–10 6 times higher than that of cyanocobalamin, was performed by Sep-Pak C 18 purification and concentration of cyanocobalamin followed by HPLC with detection at 550 nm. The method is simple, rapid, sensitive and reproducible. The calibration graph was linear in the range of 0-0.2 μg. The recovery of cyanocobalamin was over 95% by the standard addition method. There was good agreement between the cyanocobalamin concentrations indicated and found.
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