Abstract
Full-field optical coherence microscopy (FFOCM) is an interferometric technique for obtaining wide-field microscopic images deep within scattering biological samples. FFOCM has primarily been implemented in the 0.8 mum wavelength range with silicon-based cameras, which may limit penetration when imaging human tissue. In this paper, we demonstrate FFOCM at the wavelength range of 0.9 - 1.4 mum, where optical penetration into tissue is presumably greater owing to decreased scattering. Our FFOCM system, comprising a broadband spatially incoherent light source, a Linnik interferometer, and an InGaAs area scan camera, provided a detection sensitivity of 86 dB for a 2 sec imaging time and an axial resolution of 1.9 mum in water. Images of phantoms, tissue samples, and Xenopus Laevis embryos were obtained using InGaAs and silicon camera FFOCM systems, demonstrating enhanced imaging penetration at longer wavelengths.
Highlights
Full-field optical coherence microscopy (FFOCM), termed full-field optical coherence tomography (FFOCT), is an interferometric technique that utilizes spatially incoherent illumination and array detection to provide high-resolution transverse images of reflected light within biological specimens [1,2,3,4,5,6,7,8,9,10]
We report the use of Indium Gallium Arsenide (InGaAs) area scan camera in an ultrahigh transverse and axial resolution FFOCM system operating at a wavelength range of 0.9 - 1.4 μm
Differences between the measured and calculated point spread function (PSF) can be attributed to two factors: The spectral responsivity of the camera used for this calculation may be slightly different from actual spectral responsivity, and the finer spectral structures of the source may not have been resolved by our optical spectrum analyzer (0.08 nm), As a result, it is likely that the spectrum we utilized for PSF calculation was only an estimate of the true spectral content of the source
Summary
Full-field optical coherence microscopy (FFOCM), termed full-field optical coherence tomography (FFOCT), is an interferometric technique that utilizes spatially incoherent illumination and array detection to provide high-resolution transverse images of reflected light within biological specimens [1,2,3,4,5,6,7,8,9,10]. Low transverse resolution (~35 μm) wide-field OCT, which utilized collimated illumination of coherent light source (mode-locked Cr4+:forsterite laser), has showed the potential of InGaAs area cameras for full-field imaging [15]. In this manuscript, we report the use of InGaAs area scan camera in an ultrahigh transverse and axial resolution FFOCM system operating at a wavelength range of 0.9 - 1.4 μm. The penetration depth of the InGaAs system is directly compared to that provided by a similar silicon array FFOCM system in a variety of samples, including an Intralipid phantom, tissues ex vivo, and Xenopus laevis embryos
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