Abstract
Four polysaccharide fractions (CMP-1, CMP-2, CMP-3 and CMP-4) were extracted from cultured Cordyceps militaris (CM) using ultrahigh pressure extraction (UHPE), and purified using ultrafiltration, DEAE-Sepharose Fast Flow and Sephadex G-100 column chromatography. The effects on yields of crude polysaccharides (CCMP) were investigated by the response surface methodology (RSM) based on Box-Behnken Design (BBD). The optimal extraction condition for UHPE was: 5.18min, 424MPa, and 43.5°C. Under these conditions, the CCMP yield was 14.89±0.68%. The chemical characterizations of CMPs were determined by gas chromatography and high performance gel permeation chromatography (HPGPC). The antioxidant activities of CMPs were evaluated by ferric reducing antioxidant power (FRAP) and free radical scavenging assay. CMPs are composed of D-glucose, D-galactose and D-mannose, demonstrating noticeable antioxidant activities in a concentration-dependent manner. UHPE as an effective and environmental-friendly technique could be employed for extracting active ingredients from plants.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
