Ultrafiltration Fractionation of Bovine Hemoglobin Hydrolysates: Prediction of Separation Performances for Optimal Enrichment in Antimicrobial Peptide.

Sophie Beaubier,Naïma Nedjar,Romain Kapel,Rémi Przybylski,Pascal Dhulster,Alice Bodin

doi:10.3390/membranes11020073

Abstract

Hydrolysis of bovine hemoglobin (bHb), the main constituent of bovine cruor by-product, releases a natural antimicrobial peptide (NKT) which could present a major interest for food safety. To enrich this, tangential ultrafiltration can be implemented, but ultrafiltration conditions are mainly empirically established. In this context, the application of a simulation method for predicting the NKT yield and enrichment was investigated. Ultrafiltration performances were studied for decolored bHb hydrolysates at different degrees of hydrolysis (DH; 3%, 5%, 10% and 18%) and colored hydrolysates (3% and 5% DH) with 1 and 3 kg·mol−1 regenerated cellulose membranes. The simulation method helped to identify the most promising hydrolysate (in terms of NKT enrichment, yield and productivity) as the 3% DH colored hydrolysate, and UF conditions (volumetric reduction factor of 5 and 3 with 1 and 3 kg·mol−1 membrane, respectively) for higher antimicrobial recovery. A maximal enrichment factor of about 29 and NKT purity of 70% in permeate were observed. The results showed that the antimicrobial activity was in relation with the process selectivity and NKT purity. Finally, this reliable method, applied for predicting the ultrafiltration performances to enrich peptides of interest, is part of a global approach to rationally valorize protein resources from various by-products.

Highlights

Published: 20 January 2021In the food industry, bovine blood is an inevitable part of meat production and involves an environmental problem due its lack of harnessing [1]
1000 g·mol−1 from 3%DHdec to 18%DHdec. This meant that the proteolysis followed a zipper-type mechanism. This is because, during the zipper-type proteolysis, the protein is fully hydrolyzed at the early stage of the reaction into a first set of intermediate peptides which are further hydrolyzed into final and smaller peptides at a higher degree of hydrolysis [26,27]
A simulation method was applied to predict the enrichment rate, extraction yield and purity of an antimicrobial peptide (NKT) in various bovine hemoglobin hydrolysates during ultrafiltration

Summary

Introduction

Published: 20 January 2021In the food industry, bovine blood is an inevitable part of meat production and involves an environmental problem due its lack of harnessing [1]. NKT contains no hydrophobic residue, involving a particular and strong antimicrobial mechanism with no secondary structure unlike most antimicrobial peptides [4]. This peptide was previously described as a growth inhibitor of pathogenic bacteria commonly involved in the contamination phenomenon during the storage and distribution of food [5]. NKT derived from bovine hemoglobin showed antimicrobial and antioxidant properties as effective as the BHT activities after application into ground beef meat under refrigeration [6]. This peptide could be a promising natural preservative. NKT appeared early during the pepsic hydrolysis of bovine hemoglobin (bHb) and it was a final

Methods

Results

Conclusion