Abstract

Dopamine and serotonin are two neurotransmitters that play critical role in human physiology. In this work, it has been aimed to synthesize a chip-based sensing platform that can detect dopamine precisely in a non-enzymatic way in presence of a series of electroactive interfering agents. Copper oxide (CuO), a p-type material with excellent surface and electrochemical properties, has been deposited through direct current (DC) sputtering technique on indium doped tin oxide (ITO) coated glass substrates as the sensing platform. The structure, phase purity and other essential properties of the modified electrode were established through x-ray diffraction (XRD), field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM), Fourier transform infrared spectroscopy (FTIR) and ultraviolet – visible (UV–Vis) spectroscopy. Detailed electrochemical investigations with the modified electrode have also been carried out using cyclic voltammetry (CV), chronoamperometry (i – t) and electrochemical impedance spectroscopy (EIS) that revealed ultrafast response (10 ms), excellent sensitivity (5.41 µAµM-1cm−2) and low limit of detection (LoD) of 0.46 µM for the electrode towards dopamine. The electrode exhibited sensitivity of 0.37 µAµM-1cm−2 with average LoD of 19.2 µM towards serotonin, which is inferior to dopamine. The threshold response potential was also quite different for dopamine and serotonin. The modified electrode was also found to be nonresponsive to a series of electroactive interfering agents, making it dopamine selective. The role of p-type conductivity of the electrode material towards such selective detection of dopamine was also explained. The electrode was notably reliable from the point of view of electrochemical performance and structural stability. Real sample analysis was also carried out by taking urine sample from adult male human.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call