Abstract
Since the transport of fluid and weak electrolytes by the small intestine is markedly inhibited by acetazolamide we have studied the cytochemical localization of carbonic anhydrase (CAH) in rat jejunum using the erythrocyte as a model system. Pieces of jejunal tissue were glutaraldehyde-fixed, frozen to -20C and 10 micron sections were obtained with a cryostat microtome. Human erythrocytes were similarly fixed, saline-rinsed and embedded in gelatin which was then treated in the same manner as tissue.The histochemical reaction product in light microscopy sections is CoS derived from the precipitation of catalysed CoCO3 formation. The reaction time was 8 minutes and the visible CAH activity in both erythrocytes and jejunum (Fig. 1, 2 and 6) was abolished by 1 X 10-4M acetazolamide.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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