Abstract
An enzymic digestive method using either purified mould lipase or snake venom phospholipase A2 was applied to ultracytochemical observations on both newly developed lipid droplets in the parenchymal cells of Ethionine-evoked triglyceride fatty liver and characteristic cytoplasmic bodies in the hepatocytes of Coralgil-induced phospholipid fatty liver. The reaction products in the author's method indicated that PL might be synthesized in ER of the hepatic cells, that the synthesis of liver TG might be restricted to the smooth ER and TG-rich particles originating in the same smooth compartment of ER where TG would be packed into the droplets by a coat of polar lipids, PL, and that the inclusions induced by Coralgil might be digested in the participation of lysosomal enzymes of the hepatic cells and they are easily acted upon by phospholipase. This clarifies the nature of PL-rich bodies.
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