Abstract
DNA-based FluoroCubes were recently developed as a solution to photobleaching, a ubiquitous limitation of fluorescence microscopy (Niekamp, Stuurman, & Vale, Nature Methods, 2020). A FluoroCube is a compact ∼4×4×5.6 nm3 four-helix bundle of synthetic DNA that has six fluorophores attached to it. FluoroCubes exhibit remarkable photostability, remaining fluorescent up to ∼50× longer than single fluorophores under constant illumination and emitting up to ∼40× as many photons in total. This work seeks to answer two important questions about FluoroCubes: First, what is the mechanism by which photostability is enhanced? Second, how compatible are FluoroCubes with fluorescence energy transfer techniques? The answer to the first question is crucial to our understanding of FluoroCube design and the chemical physics of photobleaching, while the second question relates to the application space of FluoroCubes.
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