ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY FOR SIMULTANEOUS ESTIMATION OF ACETAMINOPHEN, DEXTROMETHORPHAN, LEVOCETIRIZINE AND PHENYL PROPONOLAMINE IN BULK AND PHARMACEUTICAL DOSAGE FORM

Abstract

A novel, rapid and stability indicatinganalytical method is developed and validated for simultaneous quantification of levocetirizen, dextromethorphan, paracetamol, and phenylpropanolaminein bulk and its Pharmaceutical formulations by UPLC a Kromosil C18 (250 x 4.6 mm, 5m).column with a solvent mixture of sodium dihydrogen phosphate :methanol(60:40 %V/V) 0.1N hydrochloric acid is used to adjusted the pH : 7.5 as mobilephasewith a flow rate of 1 ml/min. Isocratic mode was used for the separation of Levocetirizen, dextromethorphan, paracetamol, and p h e ny l p ro p a n o l a m i n e. T h e re t e n t i o n t i m e s o f L evo c e t i r i z e n , d e x t ro m e t h o r p h a n , p a ra c e t a m o l , andphenylpropanolamine were found to be around 2.5 min,5.2min,3.9,5.9minrespectively From the linearity studies the specified range for Levocetirizen was determined to be 1.25-7.5 g/ml and for dextromethorphan was determined to be 3.75-22. g/ml, and for paracetamol was determined to be 125-750 g/ml and for phenylpropanolamine was determined to be 6.25-37.5 g/ml. The proposed method was successful in simultaneous quantification of Levocetirizen, dextromethorphan, paracetamol, and phenylpropanolamine.