Ultra performance liquid chromatography atmospheric pressure photoionization high resolution mass spectrometric method for determination of multiclass pesticide residues in grape and mango juices

Abstract

A novel analytical method was developed for determination of organochlorine, synthetic pyrethroid, organophosphate and carbamate pesticide residues in fruit juices using ultra performance liquid chromatography–atmospheric pressure photoionization–high resolution mass spectrometry (UPLC–APPI–HRMS). The analytes were extracted from fruit juices by dispersive solid-phase extraction using multi-walled carbon nanotubes (MWCNTs). The analysis was carried out in full scan mode using dual ionization mode of APPI in the mass range of 100–650 units. The limit of detection and limit of quantification values for the pesticides were in the range of 0.025–0.15ngmL−1 and 0.1–0.5ngmL−1 respectively. The matrix effect of the method was found to be low and extraction recoveries were in the range of 60–110%. Some of the real fruits juice samples showed the presence of some pesticides in the range of 6.5–24.8ngL−1.